Antisense effect of oligodeoxynucleotides complementary to the mini-exon sequence of the protozoan parasite Leishmania amazonensis
Identifieur interne : 000368 ( France/Analysis ); précédent : 000367; suivant : 000369Antisense effect of oligodeoxynucleotides complementary to the mini-exon sequence of the protozoan parasite Leishmania amazonensis
Auteurs : E. Pascolo [France] ; C. Blonski [France] ; D. Shire [France] ; J. J. Toulmé [France]Source :
- Biochimie [ 0300-9084 ] ; 1993.
English descriptors
- Teeft :
- Acridine, Acridine residue, Amazonensis, Amazonensis mrna, Antisense, Antisense oligodeoxynucleotides, Antisense oligomers, Antisense oligonucleotides, Base pairs, Duplex, Gene expression, Hybrid, Hybrid stability, Intercalating, Intercalating agent, Intercalating agents, Leishmania, Leishmania amazonensis, Mrna, Nucleic, Nucleic acids, Oligodeoxynucleotides, Oligodeoxynucleotides covalently, Oligomer, Oligomers, Oligonucleotide, Oligonucleotides, Parasite, Protein synthesis, Target sequences, Thuong, Toulm6, Unmodified, Wheat germ.
Abstract
Abstract: We have targeted the mini-exon of Leishmania amazonensis, the sequence present at the 5′ end of every mRNA of this protozoan parasite, with a complementary 12-mer, either unmodified (12 Le II) or linked to an acridine derivative (12 Le II Acr). Physical measurements performed either in solution or on nitrocellulose filters showed that the two oligomers exhibited the same affinity for both DNA and RNA target sequences. Furthermore, the two oligomers 12 Le II and 12 Le II Acr inhibited in vitro translation of L amazonensis mRNAs, in a wheat germ extract, to the same extent. Those results indicated that the intercalating agent did not stabilize the duplex formed by the antisense oligomer and its target sequence.
Url:
DOI: 10.1016/0300-9084(93)90023-L
Affiliations:
- France
- Aquitaine, Midi-Pyrénées, Nouvelle-Aquitaine, Occitanie (région administrative)
- Bordeaux, Toulouse
- Université Toulouse III - Paul Sabatier
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Toulmé</name><affiliation></affiliation><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biophysique Moléculaire, INSERM CJF 90-13, Université de Bordeaux II, 146, rue Léo-Saignat, 33706 Bordeaux</wicri:regionArea><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Bordeaux</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Biochimie</title><title level="j" type="abbrev">BIOCHI</title><idno type="ISSN">0300-9084</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1993">1993</date><biblScope unit="volume">75</biblScope><biblScope unit="issue">1–2</biblScope><biblScope unit="page" from="43">43</biblScope><biblScope unit="page" to="47">47</biblScope></imprint><idno type="ISSN">0300-9084</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0300-9084</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acridine</term><term>Acridine residue</term><term>Amazonensis</term><term>Amazonensis mrna</term><term>Antisense</term><term>Antisense oligodeoxynucleotides</term><term>Antisense oligomers</term><term>Antisense oligonucleotides</term><term>Base pairs</term><term>Duplex</term><term>Gene expression</term><term>Hybrid</term><term>Hybrid stability</term><term>Intercalating</term><term>Intercalating agent</term><term>Intercalating agents</term><term>Leishmania</term><term>Leishmania amazonensis</term><term>Mrna</term><term>Nucleic</term><term>Nucleic acids</term><term>Oligodeoxynucleotides</term><term>Oligodeoxynucleotides covalently</term><term>Oligomer</term><term>Oligomers</term><term>Oligonucleotide</term><term>Oligonucleotides</term><term>Parasite</term><term>Protein synthesis</term><term>Target sequences</term><term>Thuong</term><term>Toulm6</term><term>Unmodified</term><term>Wheat germ</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: We have targeted the mini-exon of Leishmania amazonensis, the sequence present at the 5′ end of every mRNA of this protozoan parasite, with a complementary 12-mer, either unmodified (12 Le II) or linked to an acridine derivative (12 Le II Acr). Physical measurements performed either in solution or on nitrocellulose filters showed that the two oligomers exhibited the same affinity for both DNA and RNA target sequences. Furthermore, the two oligomers 12 Le II and 12 Le II Acr inhibited in vitro translation of L amazonensis mRNAs, in a wheat germ extract, to the same extent. Those results indicated that the intercalating agent did not stabilize the duplex formed by the antisense oligomer and its target sequence.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Aquitaine</li><li>Midi-Pyrénées</li><li>Nouvelle-Aquitaine</li><li>Occitanie (région administrative)</li></region><settlement><li>Bordeaux</li><li>Toulouse</li></settlement><orgName><li>Université Toulouse III - Paul Sabatier</li></orgName></list><tree><country name="France"><region name="Nouvelle-Aquitaine"><name sortKey="Pascolo, E" sort="Pascolo, E" uniqKey="Pascolo E" first="E." last="Pascolo">E. 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